Ghamartaj Hossein

5 Questions 5 Answers 0 Followers

Questions related from Ghamartaj Hossein

Why an overexpressed gene is down-regulated in tumors isolated from NOD/SCID xenograft mice model?

Hi We have overexpressed a gene from Wnt family in cell lines. We further assessed tumorigenesis and metastasis in NOD/SCID xenograft model. We were surprised that our overexpressed gene was...

08 June 2020 5,817 0 View

How to do easily verteporfin photoactivation in cell culture?

Hello We are working with verteporfin (VP) and it is known that it has diverse action on cancer cells related to the presence of light. I appreciate knowing a simple way to photoactivate VP in...

11 January 2020 4,189 0 View

How to reduce autofluorescence of big cells assessed with confocal microscopy?

I am working with a breast cancer cell line which has a high autofluoresence. Does anybody can give me a clue to reduce autofloresence? I used non fixed cells for incubation with primary antibody...

09 March 2018 6,744 7 View

How clonogenocity can decrease wiyhout cell cycle alteration?

we observed lower clonogenocity in our transfected cells ( clonogenic assay was performed in adherent condition seeded at low density) but cell cycle was not altered. how we can explain this?

05 September 2017 1,273 5 View

How can I get good quality RNA from tissue despite good peak and ratios for phenoland salt contamination?

We tried to observe the quality of human RNA from tissues by agarose gel and incubation of RNA at 70C for 10 min prior to gel loading. However, we see only one spot or smear. RT-PCR of bactin was...

03 November 2015 9,308 6 View