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Questions related from Georgios Valianatos
I am planning to use the CRISPR method to delete or inactivate a specific gene in Vibrio Cholerae. Which CRISPR system is the most suitable in this case?
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I would like to knock out a specific gene of Vibrio Cholerae. I am aware of the Transflp method but I wonder whether an even more simple method exists.
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I'm thinking of using oligofectamine for transfecting cells with plasmid.
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