Geeta Kumari

3 Questions 6 Answers 0 Followers

Questions related from Geeta Kumari

Has anyone measured enzyme inhibition constant (KI) using ITC?

My drug is inhibitor and I am measuring its Ki against trypsin, but my substrate is not soluble in same buffer. If I use DMSO to dissolve that, then do I need to dialyze it with buffer as well?...

14 September 2013 6,366 3 View

Has any one done Parallel Artificial Membrane Permeation Assays (PAMPA)?

I want check the the permeability of my drug (peptide) artificially. Has anyone any idea which multipore plates are better? With underdrain removed or with underdrain?

09 September 2013 364 9 View

What is the best way to check stability of peptide against pepsin enzyme?

I wish to check the stability of peptide invitro in presence of pepsin, can any one tell me the best way?

12 August 2013 6,012 5 View