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Questions related from Gayathri Kks
For 100ml of 50mM Sodium Phosphate Buffer (pH=7), I first prepared 100ml of 50mM of sodium phosphate monobasic anhydrous (NaH2PO4) and 50mM of sodium phosphate dibasic anhydrous (Na2HPO4)...
12 December 2018 1,032 4 View
I am trying to amplify a gene using genomic DNA and Taq polymerase. I got two clear bands, one is the specific band with very low intensity and another band which is non-specific with very high...
07 July 2018 8,856 5 View
In PCR cDNA amplification using Phusion DNA polymerase, how to avoid non-specific multiple bands? *Non-specific bands are having high intensity. Give me some suggestions...
02 February 2018 9,587 5 View
Currently, I am working in plants. In RNA extraction using Trizol method, whether there is a need to add DNase and in which step I should use DNase? Is there any Standardized protocol for RNA...
01 January 2018 8,880 6 View
In the preparation of 50X TAE buffer, when should we add glacial acetic acid? BEFORE or AFTER autoclaving the buffer ?
01 January 2018 9,068 4 View
I have a gene of interest (assume X) which plays role in many developmental events in plants. But I am trying to find its role in abiotic stress sepcifically drought stress. I have checked the...
01 January 1970 9,462 4 View
