Gautham Honnavara

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Questions related from Gautham Honnavara

I can't detect any band after Batch Affinity Chromatography using MagPrep P-25 Streptavidin Beads. Can anyone help?

I am trying to elute proteins from magnetic beads by immobilizing biotinylated DNA to the beads and therefore elute proteins from the beads, but unfortunately after elution, I can't see any band....

07 July 2013 5,666 2 View

How much elution buffer should I use to elute proteins from the Streptavidin Magnetic Beads ?

I am using 33 microleter magnetic bead slurry to bind proteins..Can anyone please tell me how much elution buffer I should add so that I don't dilute my proteins and also enough so that I could...

07 July 2013 8,594 1 View