Gaurav Shoeran

3 Questions 4 Answers 0 Followers

Questions related from Gaurav Shoeran

In-solution trypsin digestion : Before reduction/Trypsin digestion is it necessary to precipitate protein from whole cell lysate or crude lysate OK?

I am performing whole proteome analysis. My protocol suggests boil whole cells in Guanidinium hydrochloride buffer then centrifuge followed by the usual reduction /alkylation and trypsin...

28 August 2019 2,417 2 View

JC1 facs gating why cells are falling in gate q1 and q3?

I am inducing apoptosis in Leishmania by heat then I stain my cells in PBS for 30 minutes at 37 degree celsius. My positive and negative control are working fine and all cells are falling in...

03 March 2019 6,593 1 View

What percentage of total proteins indentified in LCMS based proteomics acceptable for publication?

My model organism is Leishmania containing about 8000 total protein coding genes. I am performing LCMS based whole proteomics to do qualitative analysis under different conditions. What percentage...

28 February 2019 2,782 4 View