Gaurav Joshi

9 Questions 12 Answers 0 Followers

Questions related from Gaurav Joshi

How to condense the following statement in terms of molarity: "10 picomoles of H2O2 in a 100 µL volume"?

I am bit confused with how exactly to write the results in terms of molarity for the said statement (1 pmol = 1.0E-12 mol). Any suggestions and help would be really appreciable.

03 January 2019 7,792 1 View

How to synthesize N-formyl containing molecules using deuterated formic acid (DCOOH)?

I have been synthesizing N-formylated compounds previously using formic acid, but when the formic acid was replaced with deuterated formic acid reaction is not progressing under similar...

15 March 2018 9,746 4 View

What will the effect of Xanthine Oxidase Inhibition on HIF-alpha gene expression?

In my experiment i am getting increased expression of HIF-1alpha gene after the qPCR experiments. the results are consistent with allopurinol and other synthetic molecule. Kindly help me with...

20 December 2017 1,407 2 View

What are the various parameters (example H2O2 OR ROS LEVEL) that can be efficiently measured to validate effect of Xanthine Oxidase in cancer cells?

Xanthine oxidoreductase (XOR) has been implicated in the process of oncogenesis either directly because it is able to catalyze themetabolic activation of carcinogenic substances or indirectly...

05 September 2017 7,077 0 View

What is the difference between cytotoxic and antiproliferative activity with respect to cancer chemotherapeutic agents?

In the majority of studies, it is written that compound X exhibited cytotoxic activity or antiproliferative activity, which sometimes becomes confusing to get across. Therefore, a clear cut...

17 January 2016 1,539 3 View

Is it possible to reduce aromatic nitro based compounds to their respective amino groups via Nitroreductase enzymes in vitro?

I want to assess the toxicity of nitro compounds toward hypoxic mammalian cancer cells in vitro, and check out whether the nitro based compounds gets reduced to respective amines utilising the...

18 December 2015 167 4 View

Why does the Acetylation by Acetyl transferases enzymes occur majorly only at lysine, but not on histidine and arginine?

Lysine acetylation is a conserved protein post-translational modification that links acetyl-coenzyme A metabolism and cellular signalling. Recent advances in the identification and quantification...

24 November 2015 1,201 1 View

How to completely remove iron (Fe) powder from the reaction mixture?

I am basically using a mixture of iron and hydrochloride to reduce an aromatic nitro group into an amine, but the problem is that the iron is still left in the reaction mixture. I have tried...

22 May 2014 781 6 View

If I synthesize pyrazolines from chalcones using hydrazine hydrate in acetic acid medium, will I definitely get N-acetylated pyrazolines?

I just want to synthesize simple pyrazolines with no N-acetylation. Is the acetic acid method reliable to do so, or is there a preferable method to get pyrazolines other than using methanol as...

10 December 2013 8,491 4 View