Gaston Amzati

4 Questions 6 Answers 0 Followers

Questions related from Gaston Amzati

ARLEQUIN: ΦST or FST fixation index?

ΦST or FST fixation index?I'm looking for genetic differentiation between 6 different populations of ticks. I'm using the cytochrome oxydase C subunit I (COI) haplotype data in ARLEQUIN. Can...

03 March 2017 4,050 1 View

How to trim DNA sequences at the same length (Uniform size) ?

Hello, I trimmed and assembled (Reverse and Forward) my sequences using CLC Main Workbench software. The trimming I did aims to remove poor quality 3’ and 5’. I’m going to perform multiple...

04 April 2016 9,942 3 View

Does using same primer set twice in a nested PCR make sense?

is there a problem with running a PCR and then running the same system again, with the same primers, with the product of the first reaction to further amplify the product? I m using a nested PCR...

08 August 2015 317 8 View

Does anyone know a published paper or book with the mutation rate of Cytochrome Subunit I Gene (COI) for Ixodid ticks?

I'm using COI sequences for genetic populations and historical demography studies of the hard tick Rhipicephalus appendiculatus. I would like to estimate the time of demographic expansions and...

01 January 1970 6,299 2 View