Gareth Whiteley

5 Questions 13 Answers 0 Followers

Questions related from Gareth Whiteley

Quantifying micro RNA (miRNA) from non-model organisms when the concentration is below the dynamic range of common quantification techniques?

Hello, Can anyone suggest a method for quantifying micro RNA (miRNA) when the concentration is below the dynamic range of common quantification techniques such as Qubit and the Bioanalyzer. Any...

08 August 2015 423 4 View

What does ‘coupling of ligands containing primary amines’ mean for NHS-activated HP columns (17-0716-01; GE)?

I have acquired some NHS-activated HP columns (17-0716-01; GE), which are described for ‘easy coupling of ligands containing primary amines’. What does that actually mean? I am under the...

07 July 2015 9,892 6 View

What online tool can I use to generate protein motifs/profiles from multiple alignments?

I would like to generate protein motifs/profiles/regular-expressions from multiple sequence alignments. Can anybody please suggest a tool for such? Preferably online and not command line.

04 April 2014 1,384 7 View

Does anyone have experience in sending PCR amplified cDNA for Illumina (MiSeq) sequencing? Are there any potential issues I should be aware of?

If you do not have sufficient mRNA for Illumina transcriptome sequencing (e.g.

04 April 2014 4,141 2 View

Storage of total RNA as an ethanol precipitate?

I have a couple of quick questions about storage of total RNA as an ethanol precipitate. I have 30ul of total RNA in H20 at about 2000ng/ul. Should I make this 30ul up to 0.3M Sodium acetate (by...

03 March 2014 3,338 20 View