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Questions related from Garam Wi
Hello everyone, thesedays i have serious problem in cloning. I usually do T-vector cloning with insert before I ligase into expression vector.T-vector cloning is comfirmed by sequencing but the...
11 November 2016 5,787 5 View
I wanna do haemagglutination assay, but I don't know what should i choose the RBC for this experiment. I'm going to do animal experiments using mice and then I will analyze their antibody...
07 July 2016 3,812 6 View
Hello everyone, I have a question about recombinant e.coli culture. At my last experiments, I found that my recombinant e.coli expressed target protein very well. so I wanted to increase their...
06 June 2016 7,558 4 View
I extracted plasmid from ETEC and positive control(DH5a), and there were no problem. And then enzyme digestion. but I found that the positive control plasmid was digested perfectly, but I couldn't...
06 June 2016 4,263 7 View
I expressed three proteins(ex.A, B and C) in enterotoxigenic e.coli except C with 0.5mM IPTG. (C is expressed in other bactera) and then I checked it using commassie brilient blue staining and...
05 May 2016 4,458 7 View
Well, I want to express protein using OmpA on bacteria surface(outer membrane). so I extracted e.coli DNA from ETEC and then PCR to get OmpA. originally, I expected that the sequence of OmpA is...
05 May 2016 6,673 0 View
