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Questions related from Gaoqian Feng
Hi all We imported a tube of hybridoma cell line, but the courier company accidentally forgot to top up dry ice, while my cells were kept at -10 degree for approx 2 days. I tried to put the cells...
18 April 2024 3,104 2 View
Hi We recently started our insectary with A. Stephensi mosquitos, but we had very few larvae progress into pupae stage, while the majority stuck at larvae stage for weeks. They don't die, just...
27 December 2023 1,619 1 View
Hi all I need a method to isolate mosquito cell membrane protein from whole mosquito abdomen. Therefore, I will need to remove the mosquito hemolymph and exoskeleton. I have not find a clear...
05 February 2021 5,912 2 View
Hi all I was trying to perform a ELISA, in which I coat the plate with strep, then add in biotin conjugated peptide, then human serum and use anti-human IgG to detect antibody level to the...
21 August 2019 4,708 3 View
Hi all I recently used a FITC conjugated antibody in a ELISA as secondary antibody followed by anti-goat-HRP as tertiary antibody. However, I got quite week signal in relative to background (the...
24 April 2018 5,404 3 View
We recently received some Plasmodium falciparum sporozoites cryovials which are frozen with HES (hydroxy ethyl starch),Trehalose and DOPC (Dioleoylphosphatidyl-Choline) based freezing solution. As...
27 July 2015 9,848 1 View
Hi All I recently have got some hybridoma to generate mouse mAb to a antigen of interest. However we found the mAbs produced are IgG1, while we would like to have IgG2a. Is there anyway to work...
22 July 2014 9,892 2 View
