Gamze Guney Eskiler

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Questions related from Gamze Guney Eskiler

What is the best RNA isolation method from exosomes of serum samples ?

Hi researchers, I have to perform an RNA extraction from isolated exosomes of serum samples. I tired trizol. However, the concentration of RNA is so low and I can not perform RT-PCR analysis for...

13 November 2020 9,434 7 View

How interpret excess over bliss calculator results?

I determined the cytotoxic effects of two drugs ( A and B, 24h, 48h, 72h). The concentrations of A drug was 12,5 , 25, 50, 100 µM and B drug was 0,1, 0,5, 1, 5 and 10 µM. I used excess over bliss...

02 January 2017 4,355 1 View

Is it possible that one can detect a protein by immunofloroscence but not in western blotting?

My protein of interest gets detected in IF, but not by western. Protein is not expressed in cancer cells as the gene is carried the frameshift mutation. However, I saw ımmunofloroscence of the...

25 October 2016 4,954 3 View

How calculate Rhodamine 123 florescence result?

I had florescence Rhodamine 123 results for my control and experiment groups including cancer cells, resistant cells and control cells treated with different drug concentrations. I also measured...

01 January 1970 4,143 3 View