Gael Garcia

7 Questions 3 Answers 0 Followers

Questions related from Gael Garcia

Nanodrop One Protein A280 + Acclaro correction to measure the concentration of a CITE-seq antibody-oligo conjugate?

I conjugated some antibodies to oligos for CITE-seq. I need to determine the final concentration of my antibodies. Using the NanodropOne's Protein A280 detects that there is DNA contamination (as...

29 April 2022 8,177 2 View

How long and how often is it worth measuring sucrose preference upon administration of a subanesthetic ketamine dose in a mouse model of depression?

I am developing a sucrose preference assay as an additional measure of the effectiveness of ketamine as an antidepressant when administered at sub-anesthetic doses in several mouse models of...

22 June 2017 1,167 0 View

Is it possible to excite Alexa 350 fluorophore with the 405 diode/laser?

Our confocal microscope doesn't have a 350 laser -- the closest thing is 405. Is this at all possible? Are there any laser confocals with 350 lasers, or does it have to be done with a white light...

13 December 2016 9,970 4 View

Does anyone know of an available antiflavivirus / anti-Zika virus conjugated (labelled) antibody?

We've been using the Millipore antibody against E protein for the flavivirus group, but I want to do some FACS and was wondering if there's a pre-conjugated version out there. Thank you!

08 June 2016 3,935 3 View

Will using DAPI help me indentify and exclude debris in fixed cells FACS?

I'm carrying out a FACS assay using intracellular markers, for which I am using fixed cells (2% PFA) and permeabilized with Triton X. I'm having trouble identifying and gating out debris in my...

24 May 2016 9,892 6 View

Is there a good site or software to see if a primer pair spans an exon junction?

Hi all, I am going to perform some RT-qPCR tests to validate an experiment. I'm currently in the process of ordering primers, and I would like to get them from the Harvard Primer Bank since these...

12 August 2015 8,017 8 View

Any advice on extracting RNA and performing RT-qPCR from very few cells ( ~5,000) ?

Hi all, I am currently conducting an experiment which involves FACS-sorting a specific population of cells using an antibody of interest. In order to validate the type of cells I have collected...

20 July 2015 8,975 6 View