G. Shankar

3 Questions 2 Answers 0 Followers

Questions related from G. Shankar

How to find out (in-silico) the binding position of miRNA within the mRNA sequence of target gene ??

I have a sequence of miRNA and a large sequence of target mRNA of Arabidopsis and i want to find out the potential binding position of this miRNA within the mRNA sequence. Can anyone suggest/guide...

10 June 2020 7,960 4 View

How to confirm agrobacterium harboring pBI121 plasmid (without GUS) transformation in callus ??

I have Agrobacterium (AGL1) harboring pBI121 which contain gene of interest, it has been utilized in callus transformation, now i want confirm the transformation in early stage to ensure further...

04 June 2020 6,382 6 View

How can i calculate the required number of bacterial colonies for a 20kb insert in vector ?

A vector can accept an insert of 20 kb size and the recombinant vector can be replicated in E. coli. In order to make complete gene library of E. coli by using this vector, what is the minimum...

24 February 2020 8,733 1 View