Friedrich Wilhelm Uthe

2 Questions 7 Answers 0 Followers

Questions related from Friedrich Wilhelm Uthe

SUnSET assay in puromycin resistant cells?

Has anyone tried the SUnSET assay with Puro-resistant cells? Is it possible? Could you share the protocol, if it needs adaption for that case,...

05 May 2016 4,941 1 View

Linearization of plasmid DNA before stable transfection - blunt end or sticky end? Does it make the difference in the end?

I want to linearize a plasmid and just realized that there is no blunt end restriction site available in a non-relevant plasmid area. Do you think it is relevant if I cut the plasmid blunt or...

01 January 2013 6,709 3 View