Franco Pascucci

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Questions related from Franco Pascucci

Hello everyone! I wanted to know if anyone has experience in determining sites of ubiquitination of a protein.someone has some protocol or suggestion?

Hello everyone! I wanted to know if anyone has experience in determining sites of ubiquitination of a protein, my idea is to transfect flag-protein + HA-ubiquitin, immunoprecipitate with a-flag,...

06 June 2018 712 1 View

How many RNAse H I need to add to the hibrid DNA (80 pb)- RNA (21 pb) 20 mM in 200 uL of Tris 100 mM pH8 and Mg2+ 10 mM T=35 C?

The idea of my project is: I ve made a sytem of one DNA which hybirizes with tho DNA with Cy3 and Cy5 each one (initial complex), when I add a fourth sequence of DNA at pH6 a triplex structure...

05 May 2016 2,506 0 View