Francisca Peña

3 Questions 1 Answers 0 Followers

Questions related from Francisca Peña

Why does the baseline tend to rise when I do a calcium record with Fura 2?

Good afternoon, I am writing because I am recording with Fura-2AM to observe the response to intracellular calcium to different compounds. I observe the response at two different times, at 60...

22 May 2021 9,269 1 View

Why am I not getting colonies using the Agilent QuikChange II kit?

Hello everyone, I hope you are very well. I am performing site-directed mutations with the QuikChange II kit. My first ones have a length of 50bp and a Tm of between 75 and 81 approximately. I...

10 November 2020 427 2 View

Why does the background of the image turn blue when I'm recording calcium?

Hi everyone, For some time I have been conducting calcium uptake assays in an immortalized line of astrocytes, I use the 5 μM Fura-2AM probe, I place these cells in glass covers and record for 12...

29 September 2020 3,168 3 View