Francesco Natale

2 Questions 12 Answers 0 Followers

Questions related from Francesco Natale

Has anybody experienced the loss of IPTG-induced protein while scaling up from small to large volume cultures?

I have successfully performed in the past the purification of IPTG-induced proteins from 200-500 mL cultures, following a rather standard approach, which I summarized as follows: - The E. coli...

12 December 2014 6,309 10 View

Has anybody found a reliable way to employ ELISA-based DNA methylation quantitation kits?

I am starting a project related to DNA methylation changes after exposure to certain external stimuli. As pilot experiment I would like to test the response of several cell lines using the...

02 February 2013 1,289 13 View