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Hi all, I am currently struggling to obtain a high number of immature DC from bone marrow progenitors. For example, from 4 tibias and 4 femurs (Balb/c female) I can have around 55x10^6...
03 March 2018 2,584 8 View
Hi all, I am about to start culturing THP-1 cells, and am looking for suggestion regarding the addition of antibiotics (pen/strepto) to the culture medium (which should be: RPMI 1640 + 2mM...
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Hello, I have been finding different protocols for culturing murine BMDCs. Researchers sometimes use different plates for culturing them (i.e. Petri dishes, non-TC treated, TC grade treated;...
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