Francesca Bertino

3 Questions 2 Answers 0 Followers

Questions related from Francesca Bertino

Would you use HeLa cells to find interactors of a specific protein?

I'm adding a tag to a protein with crisprCas9 in order find its interactors and localization. I'm thinking to use HeLa cells but they have a strange karyotype..what type of cells would you use...

03 March 2019 6,171 3 View

Can translation start from a mutant AUG?

Hello everyone, i'm studying a mutation occuring on the first translational start site of a gene. When performing WB i see the production of a shorter protein (translation starts from a downstream...

01 January 2019 3,219 3 View

Hi, how can I get an efficient infection of SH-SY5y neuroblastoma cell line with plvx-puro vector?

I am trying to infect SH-SY5Y with the pLVX-puro vector (lentiviral vector): I'm using as a control the empty vector while the other one is an overexpressing vector (i'm performing the infection...

12 December 2017 809 2 View