Frances Phalan

4 Questions 5 Answers 0 Followers

Questions related from Frances Phalan

Can anyone recommend a good DNA ladder that can be used for products from 100bp to 20kb?

I am looking for an easy to read ladder especially on the upper end. I have a ladder that currently goes up to 12kb but after 5kb the bands are hard to decipher.

14 January 2016 3,268 3 View

Is it necessary to stop a ligation reaction before transformation?

If so what is the best method?

04 January 2016 8,625 6 View

What is a good, reasonably priced Taq for doing colony PCR for bacterial screens?

I am doing a lot of cloning at the moment and am screening my colonies by PCR. Is it necessary to use a proofreading ploymerase? Does anyone have a recommendation for something reliable and cheap?

28 December 2015 3,135 4 View

Why are my restriction Enzyme digests not working?

I am having issues with my restriction digests and I'm not sure if it is my enzymes, my DNA template or ME! I am setting up a 50ul reaction as follows: 5ul Required buffer, 1ul enzyme, 1ug...

19 November 2015 3,637 4 View