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Hi all, I am performing an IP using 20AA biotinylated peptides as bait and recovering peptide-protein complexes using streptavidin beads. I elute the proteins using heat, SDS and mercaptoethanol,...
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Hi, I wish to perform western blot analysis to detect some proteins under hypoxia and normoxia. I cannot use b-actin as my control marker as these levels tend to change under hypoxia. Can anyone...
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