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Hi all, I am performing an IP using 20AA biotinylated peptides as bait and recovering peptide-protein complexes using streptavidin beads. I elute the proteins using heat, SDS and mercaptoethanol,...
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I am currently performing IHC for human tissue microarrays but all my tissues, including negative control where I did not add any primary antibody, stained positive. I block peroxidase with H2O2...
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Hi I am trying to find information on the thermal degradation products of tween 20 and span 20. I want to know the degradation temperature and products and are these compounds safe to inhale? Thanks
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Hi I recently performed some western blotting and have noticed that my treatment has reduced B-actin expression in cells.Do certain drugs affect B-actin expression? This result has been consistent...
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Hi, I wish to perform western blot analysis to detect some proteins under hypoxia and normoxia. I cannot use b-actin as my control marker as these levels tend to change under hypoxia. Can anyone...
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