Flor De Witte

3 Questions 1 Answers 0 Followers

Questions related from Flor De Witte

Quantifying indicators for neural growth/differentiation?

I need to compare neurons that have grown in a scaffold versus plain fluid culture, in a tissue engineering context. What are good markers that can be visualized/measured that indicate that the...

08 January 2022 2,038 0 View

What to do when you have a low Ct but a bad amplification curve in qPCR?

We're trying to develop an assay that differentiates species with specific primers, Our non-specific amplification amplifies way less than our specific amplification. However, we want a high Ct...

27 April 2021 6,813 3 View

Can you deduce amplicon size from amplification curve?

hello, I am a biochemistry student and I'm trying to optimize a qPCR assay. I have newly designed primers and they should amplify a 150 bp piece of DNA ( checked by software). I have amplification...

27 March 2021 8,939 3 View