Fiona Cunningham

3 Questions 3 Answers 0 Followers

Questions related from Fiona Cunningham

MTT Assay - Should cells be in cell incubator during incubation with MTT or at 37oC on a shaker?

I am performing an MTT assay and am wondering if the cells should be kept in their incubator during the 4hr MTT incubation, or is it ok to have them on a shaker at 37oC instead (not in a tissue...

12 March 2018 6,954 3 View

Storing fixed cell monolayers on Tranwell membrane inserts for immunofluorescence?

I am wondering if you can fix cells on transwell membranes (methanol:acetone fixation protocol) and store the fixed membranes before proceeding with the staining protocol?

16 February 2018 7,284 2 View

Is it possible to prepare SDS Reducing Buffer without including the bromophenol blue?

Hi all, I am doing an SDS-PAGE (Laemmli) gel electrophoresis but don't have any bromophenol blue to make up my SDS sample buffer. Is it possible to leave this out if you are confident of how...

27 January 2018 2,307 3 View