Fiona Cunningham

2 Questions 3 Answers 0 Followers

Questions related from Fiona Cunningham

Storing fixed cell monolayers on Tranwell membrane inserts for immunofluorescence?

I am wondering if you can fix cells on transwell membranes (methanol:acetone fixation protocol) and store the fixed membranes before proceeding with the staining protocol?

16 February 2018 7,232 2 View

Is it possible to prepare SDS Reducing Buffer without including the bromophenol blue?

Hi all, I am doing an SDS-PAGE (Laemmli) gel electrophoresis but don't have any bromophenol blue to make up my SDS sample buffer. Is it possible to leave this out if you are confident of how...

27 January 2018 2,220 3 View