Filipa Martins Cunha

6 Questions 25 Answers 0 Followers

Questions related from Filipa Martins Cunha

Unexpected bands from Plasmidic DNA ( pBluescriptII) and genomic DNA (Lactobacillus) , what are they ?

Hi everyone, I need some help on the identification of unexpected bands. Extraction of plasmidic DNA from pBluescriptII (lane 1,6) and extraction of genomic DNA from Lactobacillus plantarum...

19 October 2022 7,035 4 View

How to get rid of casein from whey fraction ?

I tried to separate casein from whey proteins by isoelectric precipitation (pH 4.6) using 10% HCl. Then, with the whey fraction, I tried to separate lactalbumin from lactoglobulin with different...

14 December 2019 1,341 5 View

Does anyone knows what is the concentration of DAB used to study kinetic activity of peroxidase ?

I read 3.5mM or 5.5mM of DAB. In 3ml of reaction use 1ml of DAB 3.5mM , 1ml of H2O2 3.5mM and 1ml of 0.1M phosphate buffer pH 6.2. Are these concentrations good for kinetic activity of...

03 February 2019 1,062 0 View

Anyone working with LC-MS/MS (Triple-TOF 5600) ?

I need same advice in blood sample preparation, enrichment. Which type of column do you use?

01 March 2018 8,623 8 View

Has anyone got more than 1 band when using abcam Rabbit anti-human CXCR4 polyclonal antibodies?

On Abcam datasheet says when using ab2090 or ab2074 there is only 1 band detected around 43kDa. They tested the antibody in HeLa whole cell lysate. I used ab2074 in HUVEC and HMEC whole cell...

07 December 2015 4,955 4 View

Is there any method to determine the energy value (Hz) of a protein?

Given that a protein is made up of atoms and atoms possess electric charge that means that the protein has a final charge, ie, has an energy. Is there any method to determine the energy value...

14 December 2011 501 17 View