How is the best way to lable TFH cells for flow cytometry?

I'm trying to study the induction of TFH cells in a vaccine protocol. I will label murine spleen and Limph nodes to detect TFH cells using aCD4, aCD279, aCD19, aCD3 and CXCR5. Which Is the best...

03 March 2016 2,701 3 View