Fernando Lindenberg

5 Questions 1 Answers 0 Followers

Questions related from Fernando Lindenberg

How can two copies of a gene turn petunias white, since two copies do not make complementary mRNA?

Jorgensen in 1986 working with petunias stumbled across RNAi. He inserted transgenic copies of chalcone synthase, a limiting enzyme in the synthesis of the flower's purple pigment, but instead of...

14 December 2014 6,465 3 View

Is anyone familiar with cloning vectors?

I am cloning a gene into a vector that has the reporter gene LacZ, but I am getting some background and almost no clones. If I use another enzyme, to cut the reporter gene LacZ, would it be a good...

10 October 2014 10,040 1 View

What is the difference between Ampligase® and Taq ligase?

I will try LCR (ligase cycling reaction) and I need a termostable ligase. I would like to know if there is a significant diffence between Ampligase® and Taq ligase. Thanks.

10 October 2014 3,449 1 View

Is anyone familiar with cloning vectors?

I am cloning a gene into a vector that has the reporter gene LacZ, but I am getting some background and almost no clones. If I use another enzyme, to cut the reporter gene LacZ, would it be a good...

08 October 2014 2,853 1 View

Is there a B. subtilis strain that can be used for aiiA gene knockout?

I am working wth B. subtilis and I would like to know if there is a B. subtilis strain that is a aiiA gene knockout, or a good protocol I could use to perform it. Thanks a lot.

08 August 2014 1,696 1 View