Fernando Lillo

2 Questions 1 Answers 0 Followers

Questions related from Fernando Lillo

Upregulation of a miRNA after its inhibition?

I am trying to inhibit one miRNA in MDA-MB-231 breast cancer cells using mirVana inhibitors (Thermo Fisher) and lipofectamine. I see efficient transfection since I in parallel transfect a...

11 November 2018 4,137 2 View

Has anyone tried plasmid transfection with lipofectamine 3000 in a melanoma human cell line or another human cell line?

Hi, I need to transfect a melanoma cell line with a plasmid but I’m not sure how much DNA (ug) is necessary for the transfection and which plate is better for this, 96, 24 or 6-well plate. I...

10 October 2015 7,531 3 View