Fernando Aleman

2 Questions 26 Answers 0 Followers

Questions related from Fernando Aleman

Transcription factor binding affinity. Method to asses binding affinity changes due to a TF interactor?

I have an interaction between a soluble protein and a Transcription Factor. Using Yeast 1 Hybrid I observed that the interacting protein of my TF can change the activity of the TF. Using 2...

09 September 2014 840 10 View

Why is enzymatic activity usually pH dependent? Why can pH increase/decrease the activity of an enzyme, and how? (please read comments first)

I have a recombinant enzyme with a given activity at pH 7.5 and 8 (highest activity). At pH5.5 and 6.5 it has lower activity (one tenth more or less). If I mix this enzyme with another protein, I...

04 April 2014 2,328 88 View