Fernanda Muckler

3 Questions 3 Answers 0 Followers

Questions related from Fernanda Muckler

Can I clone a Real-time (qPCR) product through a commercial plasmid?

I need to clone the samples used as a positive control for different RT-qPCRs. We usually use the AgPath-ID OneStep kit for RT-PCR, and TOPO TA 2.1 plasmid vector for cloning. My question is, can...

14 April 2025 7,084 5 View

How to handle CaCo-2 cell lineage?

I'm new in handling CaCo-2, as I'm using it for my undergraduation conclusion project in Virology. I'm facing some issues with this cell, and I would like some advices. I've already checked it's...

14 April 2025 8,034 0 View

Problems with Caco-2: After freezing Caco-2 cells that were working just fine, I'm not managing to thaw them anymore. What is going wrong?

I got a flask of Caco-2 to expand, and they were growing well using DMEM High with 20% FBS. I freezed them at ~1 mi. cells/mL of freezing solution (90% FBS, 10% DMSO), kept it at -80°C for a day...

31 January 2025 9,869 3 View