Felix Lichtenegger

3 Questions 7 Answers 0 Followers

Questions related from Felix Lichtenegger

What is a good method for proving cytosolic localization in confocal microscopy?

I am currently analyzing some kind of fluorescence-labeled particles that enter cells (e.g. HeLa cells), probably via endocytosis, and I need to know where they end up (endosomes vs. cytoplasm vs....

16 September 2013 7,569 14 View

Unspecific stimulation with PMA + ionomycin vs. PHA for intracellular cytokine staining of T cells

I am trying to compare cytokine production (gamma interferon and TNF alpha) by T cells in different patient populations, looking for functionality versus exhaustion. As I do not have a specific...

11 January 2013 2,056 51 View

What is a good functional assay for T cell exhaustion?

I would like to measure T cell exhaustion in an AML setting. What is a good FUNCTIONAL assay to monitor that besides phenotypic analysis (PD-1, Tim-3)?

19 December 2011 1,165 1 View