Federica Masieri

4 Questions 9 Answers 0 Followers

Questions related from Federica Masieri

How to use hyaluronic acid as a coating surface?

I would like to use hyaluronic acid as a coating surface instead of collagen or gelatine. Specifically, I would like to try to facilitate the attachment of MSC derived from hESCs. They still...

09 September 2013 4,753 5 View

Is there anyone who has experiences with the new Scorecard Technology by LiFe Technologies?

I am really intrigued by this new approach and I am considering to adopt it. It seems very reliable and accurate. What are the main advantages you found, compared to other methodologies for gene...

09 September 2013 4,458 1 View

What is your experience with gelatin coating for cell culture?

I've been using gelatin coating standard procedures for a while, to enhance attachment/differentiation of embryonic bodies and to induce the attachment of "fussy" mesenchymal stem cells. I...

07 July 2013 4,724 13 View

Does anyone have experience in using conditioned media in cell lines? What are your experiences and recommendations?

I want to try to culture a osteoblast cell line with medium from a chondrocyte cell line and vice-versa. Any specific parameter to take in the account? Does the "exausted" media need to be...

04 April 2013 6,584 31 View