Federica Foglietta

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Questions related from Federica Foglietta

Which media is the best for HUVEC cell growth?

I'm growing HUVEC cells in complete media called Endothelial Cell Growth Medium 2 (PromoCell) supplemented with Endothelial Cell Growth Medium 2 SupplementMix, as suggested by the company....

30 March 2021 7,918 4 View

Is it possible to boil protein twice before using it in Western-Blot?

I extract protein form tissue sample following AllPrep DNA/RNA/Protein Mini Kit (Qiagen). I dissolve the protein pellet with 100ul SDS 5%. Then I boil at 95°C for 5 min and after that I centrifuge...

03 September 2014 4,843 9 View