Fazileh Esmaeili

5 Questions 5 Answers 0 Followers

Questions related from Fazileh Esmaeili

HRM vs HRM with pre-amplification in Rotor-Gene software?

Dear all, I wanted to run HRM ( High Resolution Melting) using Corbett Rotor-Gene 6000 Real Time PCR Machine. When I opened the HRM folder of software , there was two option for running including...

03 January 2021 3,971 6 View

How to improve RNA quality for NGS?

Dear all, I am trying to isolate RNA for the NGS analysis. I have used trizol method. But the 260/230 of the RNAs is not good (1/2). I have attached the RNA gel image. Please have a look at the...

01 August 2018 6,671 3 View

Find the best RNA extraction time?

Dear all, My aim is investigating the effect of a plant extract on gene expression pattern in cancer cell lines based on RNA-seq. How can I find the best RNA extraction time in which genes...

25 December 2017 7,365 4 View

How can I prepare a total extract of plant? which is the best solvent ?

Dear All, I want to prepare a total extract from mint, which is the best solvent ? my purpose is study the cytotoxic effect of extract on cancer cell lines. can I use a freeze drier to remove...

07 October 2017 1,273 4 View

How should I prepare samples for ChIP-seq?

Dear All, I want to preform ChIP-seq experiment on a transcription factor and Methyl-seq for analysis of methylation patterns are there any tips that I should consider to prepare samples? Tips and...

24 July 2017 6,899 3 View