7 Questions 6 Answers 0 Followers
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Dear all, I am performing RNA-seq analysis of triticum aestivum using edgeR. I have no biological replicate. How can I find the correct dispersion value for deg analysis in edgeR?
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Dear all, I wanted to run HRM ( High Resolution Melting) using Corbett Rotor-Gene 6000 Real Time PCR Machine. When I opened the HRM folder of software , there was two option for running including...
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Dear All, I try to purify a plant compound by liquid liquid extraction. Finally, I used paraffin and the compound completely dissolved in it. For the next step, I would like to isolate the...
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Dear all, I am trying to isolate RNA for the NGS analysis. I have used trizol method. But the 260/230 of the RNAs is not good (1/2). I have attached the RNA gel image. Please have a look at the...
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Dear all, My aim is investigating the effect of a plant extract on gene expression pattern in cancer cell lines based on RNA-seq. How can I find the best RNA extraction time in which genes...
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Dear All, I want to prepare a total extract from mint, which is the best solvent ? my purpose is study the cytotoxic effect of extract on cancer cell lines. can I use a freeze drier to remove...
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Dear All, I want to preform ChIP-seq experiment on a transcription factor and Methyl-seq for analysis of methylation patterns are there any tips that I should consider to prepare samples? Tips and...
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