8 Questions 8 Answers 0 Followers
Questions related from Favio Pesaola
Hi, everybody. I'm coming today with a technical question. I'm having problems culturing HEK293 cells. Under the same culturing conditions, my HEK293T cells survive after splitting while the...
22 August 2023 6,858 8 View
Dear collegues. I am looking fora shRNA vector for silencing VAP-A protein. Does somebody has this plasmid to share an aliquot with us? Thank you very much in advance.
21 November 2018 5,435 1 View
Dear collegues, I am having serious problems trying to publish a case report. Does someone know a journal that accept a case report type paper without charges? All case reports journals I found...
13 May 2018 6,408 6 View
Dear collegues, does somebody use PEI to transfect cell lines? We are having a very low efficiency (less than 50%) and we need a higher transfection rate for the experiments. Do you know how to...
01 July 2016 7,463 10 View
We are interested to measure the lysosomal pH using some alternative at chemical fluorophores. We know there are plasmids that allow measure or estimate the lysosomal pH, for example, by the...
01 July 2016 2,207 1 View
I am studying this form of Neuronal Ceroid Lipofuscinosis and it would be very helpful to have a model cell line to work. I will be grateful for any information. Thank you very much in advance.
16 March 2016 6,608 1 View
I'm having problems with the ligation of the desired oligo and pSilencer plasmid digested with ApaI/Klenow/HindIII. I've heard that this is a difficult ligation but I've already tried more than 10...
07 August 2015 6,865 4 View
Hi. I have problems to amplify a fragment by PCR which has a high GC content. I've tried with DMSO at 3% and 5%... but nothing. I've tried modifying the conditions like temperatures and...
12 September 2014 7,489 9 View
