Fatma Hamed

4 Questions 21 Answers 0 Followers

Questions related from Fatma Hamed

Which buffer can I use to store cells overnight after Fix-perm step?

I am doing Flow-cytomteric staining of PBMCs for extra- and intra-cellular markers. I have read that I can stop the protocol after fixing and permealising the cells (fix-perm step), but I am just...

01 March 2017 7,262 4 View

Can I trust Flowcyomteric data of intracelluar cytokines without stimulating my cells?

Hi all, I am doing intracellular staining of a group of cytokines namely IFNG, IL-17A and IL-4. I haven't stimulated my cells on healthy control PBMCs hoping to see these cytokines...

18 November 2015 8,590 3 View

Can I differentiate Th1, Th2 and Th17 by cells surface receptor using Flowcytomtery?

Hi, I am doing detailed analysis of Teff in patients' peripheral blood using Flowcytomtery, Are there specific cell surface markers, which can differentiate Th1, Th2 and Th17 just to have a...

07 May 2015 6,575 2 View

Is CD45RO, CD45RA or both better for differentiation of naive, memory and activated Treg?

I am planning to characterise Treg using a large panel of markers. first I would like to subtype Treg either naïve, memory or activated, and that is just one basic question/ first step as I ll add...

18 February 2015 3,227 13 View