Fatma Hamed

3 Questions 18 Answers 0 Followers

Questions related from Fatma Hamed

Which buffer can I use to store cells overnight after Fix-perm step?

I am doing Flow-cytomteric staining of PBMCs for extra- and intra-cellular markers. I have read that I can stop the protocol after fixing and permealising the cells (fix-perm step), but I am just...

01 March 2017 7,217 4 View

Can I trust Flowcyomteric data of intracelluar cytokines without stimulating my cells?

Hi all, I am doing intracellular staining of a group of cytokines namely IFNG, IL-17A and IL-4. I haven't stimulated my cells on healthy control PBMCs hoping to see these cytokines...

18 November 2015 8,538 3 View

Is CD45RO, CD45RA or both better for differentiation of naive, memory and activated Treg?

I am planning to characterise Treg using a large panel of markers. first I would like to subtype Treg either naïve, memory or activated, and that is just one basic question/ first step as I ll add...

18 February 2015 3,193 13 View