Fatemeh Maleki

3 Questions 2 Answers 0 Followers

Questions related from Fatemeh Maleki

Why is the absorbance in my manual MDA assay too high (above 2)? Could it be due to high plasma volume?

Hello everyone, I performed the manual MDA assay using 200 µL plasma, 500 µL sulfuric acid, and 200 µL phosphotungstic acid, followed by centrifugation. The pellet was then dissolved in 400 µL of...

3 weeks ago 5,944 0 View

For a manual MDA (TBA) assay, what reagent:serum ratios should I use, can TBA be dissolved in a bain-marie, and how can I enhance color intensity?

Hello everyone, I am performing the MDA assay manually. In my protocol I use sulfuric acid, phosphotungstic acid (PTA), and thiobarbituric acid (TBA). I have three specific questions: 1. What are...

4 weeks ago 1,748 1 View

How can I prevent brown pigment and clumps after acidic acetone treatment in DNPH-based carbonyl assay of RBCs?

I isolated red blood cells, washed them with phosphate buffer, then mixed them 1:1 with isotonic phosphate buffer. Aliquots were stored at –70 °C. For comparison, some samples were stored at...

last month 7,155 3 View