Farzane R.

6 Questions 9 Answers 0 Followers

Questions related from Farzane R.

In real time RT-PCR, What is the best way to measure the expresion changes of very low expressed genes ?

I am going to measure the expression changes of some genes with low mRNA copy number by using SyBR Green. I had Ct of 30 in control group and around 35 in treatment groups. Could you please help...

07 July 2016 3,861 5 View

Is there any long lasting synthetic norepinephrine for research usage?

Hello guys, I need to assess norepinephrine chronic effect and I am searching some synthetic NE which have long lasting effect so I can inject that only a few time in each day Could you please...

03 March 2016 2,352 4 View

Is there any problem to pool all samples of cDNA from the same groups in single tube in real time PCR by sybr green?

I am thinking of the best way to reduce subject errors in real time PCR by SYBR green. I am looking quantitative gene expression differences between my four groups of rats. some of these genes...

01 January 2016 9,914 12 View

How important is considering the exon-exon junction for primer design in order to perform RT-real time PCR by SYBR-green?

I am going to measure alternations in my favorite gene expression by RT-real time PCR.(I extract RNA; prepare cDNA and perform real-time PCR) Can anyone help me to find which factor is more...

09 September 2015 2,151 11 View

Does anyone know how we can induce experimental model for circadian rhythm disturbance in rats by light?

I know about LL and DD condition which can disturb normal circadian cycle of light. when we are talking about rat as a model, how much change is ok in light/dark cycle of animal ? I mean, is it...

02 February 2015 1,882 4 View

Can anyone help me find a perfect model of Alzheimer disease?

I know there are transgenic animal models with on-off switching genes, but I am looking for a simple model which introduces complete Alzheimer symptoms without any gene manipulation. Is beta...

05 May 2014 8,724 15 View