Faruk Abdullah Saurav

5 Questions 5 Answers 0 Followers

Questions related from Faruk Abdullah Saurav

What can be the reason for CD63 and CD81 were detected by western blot but not by ms analysis.?

Hi, I isolated exosome to perform proteomic analysis by PEG precipitation method, I can see exosome like vesicles in SEM/TEM with in the size range, I have done western blot with CD63, 81, 9,...

20 November 2017 6,003 3 View

The number of exosomes increased in the culture media at stationary growth phase of cells?

I cultured three cancer cells to see the number of exosomes they release at different time intervals. The number of exosomes were increasing with the increase number of cells but when the cells...

25 October 2016 9,741 1 View

Can anyone suggest a good protocol to extract exosomal protein?

I am isolating exosome from cancer cells and doing SEM/TEM pretty well. But I am finding it very hard to extract protein from exosomes. Can anyone suggest a good protocol where I can extract total...

09 March 2016 9,523 4 View

Why my 2D gel showing so low number of bands?

I am trying to do 2D gel on mammalian cell protein. I extract the protein by ripa buffer and then do tca-acetone precipitation and then load the protein (200ug) onto the gel and continue with...

23 February 2016 1,908 3 View

How can you improve your 2D gel?

I am trying to run 2D gel. I am extracting protein from mammalian cell by using RIPA lysis buffer and then precipitate the protein with acetonitrile, then re suspend the protein in 250μl of...

04 February 2016 3,589 2 View