Fardous El-Senduny

4 Questions 23 Answers 0 Followers

Questions related from Fardous El-Senduny

Can anyone help with an explanation to Apaf-1 detection in response to drug treatment?

I treated the ovarian cancer cell line with drug#1 and with combination of drug#1 and drug#2 . I detected the Apaf-1 in the whole cell lysate. I were able to detect the band in control and after...

03 March 2014 4,345 0 View

Why after centrifugation at 10 000 RPM of cell lysate do I still have something in the supernatant (it is not clear)?

I am preparing cell lysate from control and treated cells with drug. I incubate the cells with RIPA buffer and PI for 60 min on ice then centrifugation for 60 min at 10 000 RPM at 4 degree....

02 February 2014 4,416 31 View

How to detect small molecular weight proteins (10, 11, 17, 20 KDa) by western blot?

I tried to detect these proteins by using 0.45µM nitrocellulose and transfer time was 90 min at 90 V

11 November 2013 6,059 26 View

How can you detect the loading control protein with your protein of interest in the same gel without loosing any quantity of proteins?

I'm trying to detect the change in the level of some proteins after treatment of the cells with a drug. In order to be sure that the low protein level is not, because I loaded less protein, I have...

10 October 2013 9,019 13 View