Farah Daou

3 Questions 7 Answers 0 Followers

Questions related from Farah Daou

Why the quantity and quality of RNA is not good although a pellet was obtained during RNA extraction using TRIzol?

We are trying to extract RNA from stem cells seeded using 0.5% collagen type 1 into bioactive glass (BGC) scaffolds. We did the extraction twice: 1. First, we added TRIzol to the scaffolds and...

13 May 2022 3,166 8 View

Can I use alkaline phosphatase assay kit (colorimetric) with culture media containing phenol red?

I recently used the ALP Assay Kit (Colorimetric) for the first time. I used it for culture media (and not cell lysates). However, I have 2 main issues: 1. The background value refers to my culture...

25 March 2022 456 2 View

How to prepare a 20 M β-glycerophosphate solution?

We are currently inducing osteogenesis, and for the osteogenic medium we need the concentration of β-glycerophosphate to be 20 mM in the final volume of the media (Vf = 50 mL). Therefore, we tried...

14 March 2022 3,301 2 View