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I work with HCE cells, primary and cell line. Both are difficult to detach. After incubating with trypsin-EDTA (0.05% trypsin, 0.02% EDTA), and tapping the flask, I manage to get most of the HCE...
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I used Cytochalasin D as an actin inhibitor in epithelial cells. Crystals formed and blocked the whole well (96-well plate) at 1 µM concentration, and formed few crystals at 0.5 µM, and no...
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