Fakhriedzwan Hj Idris

4 Questions 12 Answers 0 Followers

Questions related from Fakhriedzwan Hj Idris

Any recommendation on transfection reagent for siRNA treatment of C6/36 cells?

I'm looking for effective transfection reagent for siRNA treatment of C6/36 cells. I've used DharmaFect and Lipofectamine2000, but it gave transient knock-down of the protein or ineffective...

11 November 2019 9,198 4 View

Is there a method to minimize/prevent reversion of mutant virus to its wild type?

We developed a mutant DEN virus and serially passage the mutant in C6/36 cells. We observed a reversion occurred as early as passage 1 in the cell line. Is there any method to minimize or prevent...

08 August 2018 9,418 8 View

Plant lectis as means to study viral-receptor interaction and receptor characterization

How reliable and specific plant lectins (specific for mannose or N-acetyl glucosamine) to be used in the characterization and carbohydrate receptor profiling during viral infection?

06 June 2017 1,134 6 View

What is the best method to sterilize lysates which potentially contain peptide?

I'm doing a screening on lysates with unknown properties against virus on cell culture. I need to make sure the lysates are sterile so as not to contaminate my cell cultures. What is the best...

11 November 2015 6,747 3 View