Faheem Askari

3 Questions 9 Answers 0 Followers

Questions related from Faheem Askari

Can we change the growth medium (one or mixture) during cell culture passage with or without increasing the FBS volume?

I have been working on PC-3 and A549 cell culturing in DMEM+10%FBS for the last two weeks, and I have observed the slow growth of both cells. I need the expert's opinion on whether can I change...

09 September 2023 3,773 4 View

Thioesterification of RGD-peptide at C-terminus?

I want to modify my peptide. I have HOOC-RGD-peptide-NH2 and I want to react it with R-SH by dehydration method to get R-S-OC-RGD-peptide-NH2. I have tried to do so using MsOH and TEA in solvents...

11 April 2019 185 2 View

Hello! Can someone guide me please about following description?

I am labelling my peptide with MW 768.46 with FITC in DMF/TFA/H2O solvent mixture. After 12 hours stirring i get my product. It is a liquid mixture, i want to get solid product. Which method is...

20 February 2019 353 1 View