Fading Chen

5 Questions 13 Answers 0 Followers

Questions related from Fading Chen

How to reduce whole cell recording noise (~120 Hz) in Clampex?

Dear Fellows, I just finished setting up a new whole cell recording rig in a new room. Currently I am testing the system. With proper groundings for devices, the recording reveals a noise around...

13 December 2016 1,586 10 View

Can I increase pulse duration in theta stimulation for mouse hippocampus LTP induction?

Dear Fellows, I am trying to induce LTP in horizontal hippocampal slices (400 um) from ~8 month old mouse, stimulating CA3 shaffer collaterals and record from dentrides of CA1 pyramidal...

22 August 2016 1,936 4 View

Good strategies to mount dissected mouse hippocampus onto speciment disc for horizontal hippocampal slicings?

Hi guys, How do you mount the dissected hippocampus onto specimen disc for horizontal sectioning? Currently, I am trying to set up a rig to recording field EPSPs using mouse horizontal slices....

19 July 2016 6,607 4 View

How to choose a stimulating electrode for LTP in acute mouse hippocampal slices?

Hi guys, What kind of stimulating electrode you are using to deliver stimulation to induce LTP? I used a concentric bipolar electrode to induce evoked response in whole cell clamp mode in the...

06 July 2016 7,742 5 View

Why rat cortical culture has low mEPSCs freqency but high sEPSCs frequency at 14DIV

Does anyone have any clue why the frequency of mEPSCs is so low (

21 May 2014 7,056 8 View