Fabian Jung

5 Questions 9 Answers 0 Followers

Questions related from Fabian Jung

Is there any tool to determine most probable torsion angles after joining structures with Chimera?

I have a crystal structure of my protein which is a homo-tetramer. I attached experimentally a peptide sequence to my recombinant monomer unit of which the structure is not solved. That's why, I...

18 December 2018 8,904 3 View

What is the principle of the reversible BSPP-coated gold nanoparticles agglomeration upon NaCl addition?

When I prepare gold nanoparticles coated with negatively charges phosphine ligands (BSPP), they are stable, but aggregate upon addition of NaCl. However, this aggregation is reversible upon...

20 March 2017 6,091 3 View

How to attach a protein to gold nanoparticles via cysteine residues the best?

I need to attach a protein (carrying a N-terminal cysteine residue) to spherical gold nanoparticles in a defined ratio. Due to recombinant expression the target protein carries a HisTag as well....

23 August 2016 3,845 8 View

How to realize Gold-Thiol binding the best?

Hello everybody, I try to attach a cysteine-tagged protein to a citrate-stabilized gold nanoparticle. Which binding conditions do you suggest to obtain the highest yield of attachment of the...

22 June 2016 4,207 3 View

What are the comparative adhesion qualities of Neutravidin vs. Streptavidin?

Hello everybody, I am working with the protein streptavidin as linker for coupling experiments. Since streptavidin is very adhesive to reaction tubes (even LowBind) and quite expensive, I was...

23 February 2016 4,715 2 View