I am obtaining low qPCR efficiency for absolute quantification which is cause by higher Ct jump after 10^6 dilution.What could be the reason for that?

There is a jump in the expected Ct change when I go from dilution 10^6 to 10^5. When I prepare my standard curve, the most diluted standards have a difference of around 3 cycles, that would be...

06 September 2023 4,675 0 View