Evrim Aksu Mengeş

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Questions related from Evrim Aksu Mengeş

Why couldn't I get rid of cytosolic protein contamination in mitochondrial fraction in primary myoblasts?

I want to isolate crude mitochondria from primary myoblast cells for proteomics studies. The interesting thing is that I can enrich mitochondrial fraction without cytoplasmic contamination in...

09 January 2023 1,473 4 View

Which type of medium is appropriate to use during Antimycin A incubation; medium with or without fetal calf serum ?

I need help on my Antimycin A treatment protocol. I want to treat c2c12 cells with Antimycin A (AMA) which is an inhibitor of mitochondrial electron transport chain. During AMA incubation, which...

30 November 2015 3,810 5 View