Eve Corrie

3 Questions 4 Answers 0 Followers

Questions related from Eve Corrie

When reporting data, is it OK to pool repeated measures from individual animals or do I need to report the average of each animal?

For example, if I do 3 repeats of a recording for each animal, can I put the 3 separate repeats from all the animals in a list and do stats from there, or do I need to average the recordings for...

10 September 2019 9,192 6 View

How do I block when using a biotinylated toxin and a fluorescent streptavidin to bind it?

Hi all. I'm using a biotinylated conotoxin to attempt to visualise voltage gated calcium channels. At the moment I'm getting such high background/non-specific staining that it's impossible to see...

15 October 2018 6,730 3 View

What's the best way to store PFA fixed and cryoprotected tissue before cryosectioning?

I've got blocks of rat paw skin that have been immersion fixed in 4% PFA for 16 hours, then washed and cryoprotected in 30% sucrose. I ideally need to store these for about 6 months, before...

23 July 2018 9,417 8 View