Does drugs like Oxaliplatin and 5-FU interact with MTT to produce formazan crystals? Is it necessary to give a PBS wash before addition of MTT ?

I'm trying to find the IC50 concentration of 5-FU, oxaliplatin, and cisplatin in HT29 cells. While doing the MTT assay, the drug-treated cells (low conc. ones) showed more absorbance than the...

06 November 2023 6,431 0 View